Investigação molecular de arbovírus de interesse em saúde pública em primatas não humanos no município de Foz do Iguaçu - Paraná

Abstract

Resumo: Arbovirus sao virus transmitidos por artropodes e sao responsaveis por causar doencas emergentes e reemergentes de potencial zoonotico. As arboviroses destacadas neste estudo sao a Febre Amarela, Dengue e Chikungunya, doencas que constituem grandes problemas de saude publica no Brasil e no mundo. Acoes da vigilancia ativa como monitoramento, captura e avaliacao de primatas nao humanos assintomaticos auxiliam na deteccao precoce de circulacao viral e predicao de risco, de forma a compreender o papel destes animais como reservatorios destas arboviroses. O objetivo deste trabalho foi investigar a infeccao aguda por YFV, DENV 1-4 e CHIKV em sangue total de 67 PNH cativos e de vida livre, divididos em quatro especies no municipio de Foz do Iguacu- Parana, Brasil, a saber: Alouatta caraya (n=7), Callithrix penicillata (n=5), Leontopithecus chrysomelas (n=3) e Sapajus nigritus (n=23) cativos do Zoologico Municipal Bosque Guarani e do Zoologico Roberto Ribas Lange e Sapajus nigritus (n=29) de vida livre na Area de Protecao Permanente da Usina Hidreletrica de Itaipu Binacional, no periodo de outubro de 2019 a marco de 2020. O diagnostico molecular foi determinado pela RT-qPCR. Todas as amostras foram negativas para a presenca do fragmento alvo do RNA genomico viral de YFV, DENV1-4 e CHIKV. Os resultados negativos sugerem ausencia de infeccao aguda nos animais analisados e, portanto, nao constituiram fonte de infeccao para os vetores artropodes no momento da amostragem.

Abstract: Arboviruses are viruses transmitted by arthropods and are responsible for causing emerging and re-emerging diseases with zoonotic potential. The arboviruses highlighted in this study are Yellow Fever, Dengue and Chikungunya, diseases that constitute major public health problems in Brazil and worldwide. Active surveillance actions such as monitoring, capturing, and evaluating asymptomatic non-human primates help in the early detection of viral circulation and risk prediction, to understand the role of these animals as reservoirs of these arboviruses. The aim of this work was to investigate the acute infection by YFV, DENV 1-4 and CHIKV in whole blood from 67 free-living captive NHPs, divided into four species in the municipality of Foz do Iguacu-Parana, Brazil, namely: Alouatta caraya (n=7), Callithrix penicillata (n=5), Leontopithecus chrysomelas (n=3) and Sapajus nigritus (n=23) captives of the Municipal Bosque Guarani Zoo and the Roberto Ribas Lange Zoo and free living Sapajus nigritus (n=29) in the Permanent Protection Area of the Itaipu Binacional Hydroelectric Power Plant, from October 2019 to March 2020. Molecular diagnosis was determined by RTqPCR. All samples were negative for the presence of the YFV viral genomic RNA target fragment, DENV1-4 and CHIKV. Negative results suggest the absence of acute infection in the analyzed animals and, therefore, did not constitute a source of infection for the arthropod vectors at the time of sampling.