Antioxidant and antimicrobial activities of silver nanoparticles synthesized using lignin extracted from OPEFB as reducing agent

Abstract

Resumo: As ligninas extraídas dos OPEFB a quarto condições diferentes foram utilizadas na síntese de nanopartículas de prata. Os espectros UV-vis apresentam os picos SPR à 423-427 nm. As análises MET mostram as nanopartículas de forma esférica e de natureza policristalina. Os espectros FT-IR das AgNPs apresentam grupos funcionais correspondentes às ligninas utilizadas como agente redutor e de recobrimento. O tamanho médio de partícula das AgNPs esteve na faixa de 17,63 até 19,24 nm. Um perfil de potencial Zeta negativo para as AgNPs foi observado em meio com baixa concentração de íons. A atividade antioxidante das AgNPs poderia ser atribuída à lignina utilizada na síntese. As atividades antimicrobianas de todas as AgNPs foram testadas contra Escherichia coli e a CMI para a HAL3-L AgNP foi de 62,5 ug.mL-1

Abstract: Lignins extracted from OPEFB at four different conditions were used for synthesis of silver nanoparticles. UV–vis spectra show the SPR peak at 423-427 nm. TEM analysis exposes the spherical shape and polycrystalline nature of nanoparticles. FT-IR spectra of AgNPs show the presence of functional groups corresponding to the lignins used as reducing and capping agents. The average particle size of AgNPs ranged from 17.63 to 19.24 nm. A negative Zeta potential profile for AgNPs suspensions was exhibited in a low ion concentration medium. Antioxidant activity of AgNPs could be attributed to the lignin used in the synthesis. The antibacterial activity of all AgNPs was demonstrated against Escherichia coli and the MIC for HAL3-L AgNP was 62.5 ug.mL