Comparative investigations of two cataract-preventing drugs and of two different rebound tonometers in laboratory rats (Rattus norvegicus)

Abstract

Resumo: O sentido da visão é essencial e muito importante para a maioria dos seres vivos. Ao longo dos anos, pesquisas são desenvolvidas ao redor do mundo, no intuito de prevenir e tratar doenças oculares, com recursos tecnológicos e farmacêuticos. Ao longo dessa dissertação se discutem alguns recursos importantes para diagnóstico e tratamento de doenças como glaucoma e complicações oculares de diabetes mellitus. A dissertação é composta de dois capítulos, sendo o primeiro intitulado "Comparative evaluation of two commercially available rebound tonometers in rats: TONO-VERA VET® and TD 8000®" e o segundo "A Comparative study of the use of topical lanosterol and na oral anti-oxidant vitamin complex to prevent diabetic cataracts in rats and in dogs". No capítulo 1, comparou-se medidas de pressão intraocular em dois diferentes tonômetros Tono-Vera Vet® (TVV) e TD 8000® (TD8) em ratos. No capítulo 2, foi avaliada a progressão da opacidade da lente em ratos da raça Wistar induzidos, utilizando modelo experimental com estreptozotocina, ao diabetes mellitus, e de cães com diabetes mellitus natural, por meio de avaliação clínica oftalmológica durante tratamento com colírio contendo lanosterol ou complexo vitamínico contendo inibidor de aldose redutase (ácido alfa lipóico) baseado no medicamento disponível comercialmente Ocu-GLO®, com análise documentada e padronizada através de imagens fotográficas e análises complementares, tais como histopatologia (nos ratos). Os resultados obtidos trazem informações relevantes acerca de tonometria para diagnóstico glaucoma e tratamento preventivo para catarata diabética.

Abstract: The sense of sight is essential and very important for most living beings. Over the years, innumerous scientific investigations has been developed around the world in order to prevent and treat a variety of eye diseases using, technological and pharmaceutical resources. Throughout this dissertation, important theories for the diagnosis and treatment of diseases such as glaucoma and ocular complications of diabetes mellitus are discussed. The dissertation consists of two chapters, the first entitled "Comparative evaluation of two commercially available rebound tonometers in rats: TONO-VERA VET® and TD 8000®" and the second "Use of Lanosterol eye drops and a compounded vision supplement based on OCU-GLO® to prevent diabetic cataracts in rats and in dogs - a comparative and effectiveness study". Chapter 1 compared intraocular pressure measurements in two different tonometers in rats, Tono-Vera Vet® (TVV) and TD 8000® (TD8) in rats. In chapter 2, the progression of lens opacity was evaluated in Wistar rats induced, using an experimental model with streptozotocin, to diabetes mellitus, and in dogs with natural diabetes mellitus was evaluated and compared, in animals treated with eye drops containing lanosterol or an orally administrated vitamin complex containing aldose reductase inhibitor (alpha lipoic acid), which was based on a commercially available drug Ocu-GLO®. A standardized analysis using photographic images was performed. With the conclusions of both studies, we bring results, with relevant information about tonometry for glaucoma diagnosis and preventive treatment for diabetic cataract.