Prevalência e fatores associados a infecção por Mycoplasma ovis em caprinos do Estado da Paraíba

Abstract

Resumo: Micoplasmas hemotropicos (hemoplasmas) sao bacterias Gram-negativas, pleomorficas e que se aderem a membrana de eritrocitos de uma grande variedade de especies animais. Estao distribuidos mundialmente e diferentes especies destas bacterias ja foram descritas em animais domesticos, silvestres e seres humanos. Entretanto, estudos envolvendo a deteccao de hemoplasmas em caprinos sao escassos e ainda nao haviam sido realizados no Brasil. Assim, objetivou-se determinar a prevalencia e os fatores associados a infeccao por Mycoplasma sp. em caprinos do estado da Paraiba, Nordeste do Brasil. Um total de 158/402 (39,3%) animais foram positivos para o gene 16S rRNA de Mycoplasma sp. pela PCR. O sequenciamento genetico de 10% das amostras positivas demonstrou .99% de identidade com varias sequencias de Mycoplasma ovis depositadas no GenBank. Caprinos leiteiros (OR = 2.15; 95% IC: 1.40-3.32%; p = 0.0004) e anemicos (OR = 2.39; 95% IC: 1.54-3.71%; p = 0.00007) apresentaram maior chance de estarem infectados por M. ovis do que caprinos de corte nao anemicos. Amblyomma parvum (49/52, 94.23%) e Rhipicephalus (Boophilus) microplus (3/52, 5.77%) foram as especies de carrapatos encontradas nos animais. Este e o primeiro relato de deteccao molecular de M. ovis em caprinos na America do Sul. Palavras-chave: Hemoplasmas; Micoplasmose hemotropica; pequenos ruminantes; PCR

Abstract: Hemotropic mycoplasmas (haemoplasmas) are gram-negative pleomorphic bacterias that infect the erythrocites membrane of a wide range of animal hosts. This pathogen is worldwide distributed and different species were described infecting domestic and wild animals and also humans. However, studies involving the diagnosis of haemoplasmas in goats are scarce and had not yet been conducted in Brazil. Thus, the present work aimed to detect the prevalence and risk factors associated with the infection of Mycoplasma sp. in goats from Paraíba State, Northeastern Brazil. A total of 158/402 (39.3%) animals were positive for the 16S rRNA gene from Mycoplasma sp. by PCR. The sequencing of 10% of positive samples demonstrated a ?99% of similarity with multiple Mycoplasma ovis sequences deposited on GenBank. Dairy (OR = 2.15; 95% IC: 1.40-3.32%; p = 0.0004) and anemic goats (OR = 2.39; 95% IC: 1.54-3.71%; p = 0.00007) showed higher chances to be infected by M. ovis than non-anemic beef goats. Amblyomma parvum (49/52, 94.23%) and Rhipicephalus (Boophilus) microplus (3/52, 5.77%) were the tick species found in these animals. This is the first report of molecular detection of M. ovis in goats from South America. Keywords: Hemoplasmas; Hemotropic mycoplasmosis; small ruminants; PCR.